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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
10/02/2014 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
NUNES, J. D.; AZEVEDO, A. L. S.; PEREIRA, A. V.; PAULA, C. M. P.; CAMPOS, J. M. S.; LEDO, F. J. da S.; SANTOS, V. B. |
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Afiliação: |
J. D. NUNES; ANA LUISA SOUSA AZEVEDO, CNPGL; ANTONIO VANDER PEREIRA, CNPGL; C.M.P. PAULA, UFLA; J. M. S. CAMPOS, UFJF; FRANCISCO JOSE DA SILVA LEDO, CNPGL; V. B. SANTOS, Universidade Federal do Acre. |
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Título: |
DNA elimination in embryogenic development of Pennisetum glaucum x Pennisetum purpureum (Poaceae) hybrids. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 12, n. 4, p. 4817-4826, 2013. |
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DOI: |
https://doi.org/10.4238/2013.october.22.1 |
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Idioma: |
Inglês |
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Conteúdo: |
Interspecific hybridization between Napier grass (Pennisetum purpureum), which is widely grown in Brazil for cattle forage, and pearl millet (Pennisetum glaucum) has been used as a breeding strategy for the development of improved cultivars. However, the hybrid between these two species is sterile due to its triploid condition (2n = 3x = 21 chromosomes), which hinders its use in crop breeding programs. It is known that genomic alterations result from the hybridization process. In order to measure the loss of DNA during embryo development, we used flow cytometry to estimate the nuclear DNA content of triploid and tetraploid embryos produced by interspecific hybridization between Napier grass and pearl millet. The triploid and tetraploid hybrids had a mean DNA content of 4.99-4.87 and 5.25-4.84 pg, at 10 and 30 days after pollination, respectively. The mean reduction in DNA content was higher in the tetraploid hybrids. The flow cytometry results revealed progressive genomic instability in these triploid and tetraploid hybrids, with this instability causing significant alterations in the DNA content of the hybrids. |
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Palavras-Chave: |
Elephant grass. |
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Thesaurus Nal: |
flow cytometry; grasses; hybridization. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/96852/1/ARTIGO-Ledo.pdf
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Marc: |
LEADER 01919naa a2200253 a 4500
001 1979289
005 2024-02-05
008 2013 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.4238/2013.october.22.1$2DOI
100 1 $aNUNES, J. D.
245 $aDNA elimination in embryogenic development of Pennisetum glaucum x Pennisetum purpureum (Poaceae) hybrids.$h[electronic resource]
260 $c2013
520 $aInterspecific hybridization between Napier grass (Pennisetum purpureum), which is widely grown in Brazil for cattle forage, and pearl millet (Pennisetum glaucum) has been used as a breeding strategy for the development of improved cultivars. However, the hybrid between these two species is sterile due to its triploid condition (2n = 3x = 21 chromosomes), which hinders its use in crop breeding programs. It is known that genomic alterations result from the hybridization process. In order to measure the loss of DNA during embryo development, we used flow cytometry to estimate the nuclear DNA content of triploid and tetraploid embryos produced by interspecific hybridization between Napier grass and pearl millet. The triploid and tetraploid hybrids had a mean DNA content of 4.99-4.87 and 5.25-4.84 pg, at 10 and 30 days after pollination, respectively. The mean reduction in DNA content was higher in the tetraploid hybrids. The flow cytometry results revealed progressive genomic instability in these triploid and tetraploid hybrids, with this instability causing significant alterations in the DNA content of the hybrids.
650 $aflow cytometry
650 $agrasses
650 $ahybridization
653 $aElephant grass
700 1 $aAZEVEDO, A. L. S.
700 1 $aPEREIRA, A. V.
700 1 $aPAULA, C. M. P.
700 1 $aCAMPOS, J. M. S.
700 1 $aLEDO, F. J. da S.
700 1 $aSANTOS, V. B.
773 $tGenetics and Molecular Research$gv. 12, n. 4, p. 4817-4826, 2013.
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Embrapa Gado de Leite (CNPGL) |
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